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The purpose of the research should be to characterize the metabolites of EAM-2201 in human hepatocytes obtained in vitro and people in liver and urine specimens received in vivo from the autopsy of an EAM-2201 abuser.
Twelve metabolites of the synthetic cannabinoid, EAM-2201, had been tentatively determined in human hepatocytes in vitro, As well as in human liver and urine specimens in vivo for The very first time. There was a distinct difference in metabolism profile between the in vitro As well as in vivo benefits.
EAM-2201 was incubated with human hepatocytes for 3 h in a CO2 incubator as well as metabolites of EAM-2201 have been created. The human liver specimen was homogenized as well as metabolites have been extracted. The urine specimen was hydrolyzed first with β-glucuronidase plus the metabolites had been extracted.
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Then, the inhibitor concentration that supports 50 percent the maximal charge of inhibition (Ki) and maximal amount of enzyme inhibition (kinact) values were calculated utilizing the following equation with Enzyme Kinetics application:
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